H-2-restricted helper factor secreted by clone hybridoma cells.

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H-2-restricted helper factor secreted by clone hybridoma cells

Biological and serological characteristics of a helper factor secreted by cloned hybridoma cells was described. The factor is carrier specific and contains determinants shared with immunoglobulin VH bu does not react with V kappa- or V lambda-specific antibodies. Presence of four H-2I-controlled antigenic specificities, Ia.ml, Ia.m2, Ia.17, and Ia.m7, was detected. Hence, it is possible that bo...

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Two separate genes regulate self-Ia and carrier recognition in H-2- restricted helper factors secreted by hybridoma cells

H-2 heterologous T cell hybridomas were used to study the genetic control of dual, anti-nominal antigen and anti-self H-2 specificity of H-2 restricted T cell factors. Each of four hybridoma clones produced two helper factors. One was restricted for the Ia type of the normal T cell partner (H-2b), whereas the other was restricted for the ia type of the lymphoma partner (H-2k) of the somatic hyb...

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Antigen-specific, H-2-restricted helper T cell hybridomas

We have examined the carrier-specific helper activity of a number of antigen-specific, I region-restricted T cell hybridomas prepared in our laboratory. The hybridomas were assayed for helper activity in the presence or absence of exogenously added nonspecific factors found in the concanavalin A-activated supernatants of normal mouse spleen cells. Of six hybridomas tested, all six could stimula...

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Antigen-specific helper T cells required for dominant idiotype expression are not H-2 restricted

Two synergizing antigen-specific helper T (Th) cell populations are required for an optimal TEPC15 (T15)-dominated antiphosphorylcholine (PC) plaque- forming cell response . In these studies, the two Th cell sets are shown to differ in their requirements for recognition of self-major histocompatibility complex (MHC)-encoded determinants by testing the ability of Th cells from F(1) {arrow} paren...

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Restricted 2-factor polytopes

The optimal k-restricted 2-factor problem consists of nding, in a complete undirected graph K n , a minimum cost 2-factor (subgraph having degree 2 at every node) with all components having more than k nodes. The problem is a relaxation of the well-known symmetric travelling salesman problem, and is equivalent to it when n 2 k n ? 1. We study the k-restricted 2-factor polytope. We present a lar...

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ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1981

ISSN: 0022-1007,1540-9538

DOI: 10.1084/jem.154.3.942